Enhanced polyhydroxybutyrate production in transgenic sugarcane
نویسندگان
چکیده
منابع مشابه
Polyhydroxybutyrate production by a sugarcane growth promoter bacterium
Background The plastics derived from petrochemicals have many applications. However, public concern and environmental laws led to conservation policies and establish programs that stimulate the research and use of new products based on renewable resources. Polyhydroxyalkanoates (PHAs) are biopolymers synthesized by microorganisms under conditions of nutrient stress with similar properties to co...
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BACKGROUND Most bacteria are grown in a binary fission way meaning a bacterial cell is equally divided into two. Polyhydroxyalkanoates (PHA) can be accumulated as inclusion bodies by bacteria. The cell division way and morphology have been shown to play an important role in regulating the bacterial growth and PHA storages. RESULTS The common growth pattern of Escherichia coli was changed to m...
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We developed sugarcane plants with improved resistance to the sugarcane borer, Diatraea saccharalis (F). An expression vector pGcry1Ac0229, harboring the cry1Ac gene and the selectable marker gene, bar, was constructed. This construct was introduced into the sugarcane cultivar FN15 by particle bombardment. Transformed plantlets were identified after selection with Phosphinothricin (PPT) and Bas...
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Polyhydroxybutyrate [P(3HB)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-CoA reductase (PhaB) and polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha (Cupriavidus necator) with optimized codon usage for expression in tobacco. P(3HB) contents in the transformants (0.2mg/g dry cell weight in average) harboring the codon-optimized phaB gene was twofold ...
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It has been more than half a century that plant geneticists and breeders have been trying to assemble a combinationof genes in crop plants, in order to make them as suitable and productive as possible. Plant transformation technology incrop plants was first undertakenin the 1980s based on the ability of foreign gene integration into host plant genome andregeneration of transformed plant cells i...
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ژورنال
عنوان ژورنال: Plant Biotechnology Journal
سال: 2012
ISSN: 1467-7644
DOI: 10.1111/j.1467-7652.2012.00686.x